Identification and study of microbial derived immunogenic lipids in plant immunity.
Our work aims on the identification of novel microbial derived lipidic elicitors of plant immune signalling and their perception mechanism in Arabidopsis thaliana. In order to achieve this, we use analytical chemistry methods (chromatography, lipidomics, MS, NMR) and classical molecular biology and biochemistry methods. We are moreover interested in how plant endogenous lipid homeostasis in the plasma membrane and lipid signalling affect and contribute to plant immunity.
Elia Stahl received his PhD from the Heinrich-Heine-University (Düsseldorf, Germany) under the supervision of Jürgen Zeier. In his PhD he worked on functional and regulatory aspects of plant stress-inducible metabolic pathways. His PhD project was embedded in the CEPLAS (Cluster of Excellence on Plant Science) graduate school. He afterwards joined the laboratory of Philippe Reymond in the Department of Plant Molecular Biology (DBMV) at the University of Lausanne (Switzerland) as a postdoctoral fellow. During his PostDoc he worked on how plants perceive insect eggs and respond to insect oviposition.
Since December 2023, Elia Stahl is a CNRS Chargé de Recherche at the Laboratory of Membrane Biogenesis (LBM, UMR5200).
Throughout their lifecycle plants interact with a multitude of biotic stressors and have therefore evolved an elaborated immune system to counteract such threats. Induction of robust plant immunity relies on pathogen and herbivore recognition. Plants activate immune signalling upon perception of non-self herbivore- and pathogen-associated molecular patterns (HAMPs, PAMPs) and self-derived damage-associated molecular patterns (DAMPs), a process called pattern triggered immunity (PTI). Perception of those patterns is ensured by plasma membrane-localized pattern recognition receptors (PRRs). We previously discovered that phospholipids from eggs of the large white butterfly Pieris brassicae act as elicitors of plant immune signalling in the model plant Arabidopsis thaliana. The ubiquitous presence of lipids in biological membranes and as storage lipids of organisms which interact with plants led to the question whether lipidic compounds from different sources might be detected by plants as conserved features from potential enemies. Our research therefore aims on (i) the identification of novel microbial-derived immunogenic lipids, (ii) the study of the signalling mechanism activated in response to their perception and (iii) the perception mechanisms of those lipids in plants itself.
Selected Publications
Stahl E*,Maier LP, Reymond P. (2023). Insect egg-induced innate immunity: who benefits? PLoS Pathogens, 19:e1011072. https://doi.org/10.1371/journal.ppat.1011072
Stahl E*, Fernandez Martin A, Glauser G, Guillou MC, Aubourg S, Renou JP, Reymond P. (2022). The MIK2/SCOOP Signaling System Contributes to Arabidopsis Resistance Against Herbivory by Modulating Jasmonate and Indole Glucosinolate Biosynthesis. Frontiers in Plant Science, 13, 852808. https://doi.org/10.3389/fpls.2022.852808
Groux R, Stahl E, Gouhier-Darimont C, Kerdaffrec E, Jimenez-Sandoval P, Santiago J, Reymond P. (2021). Arabidopsis natural variation in insect egg-induced cell death reveals a role for LECTIN RECEPTOR KINASE-I.1. Plant Physiology, 180, 240-255. https://doi.org/10.1093/plphys/kiaa022
Stahl E, Brillatz T, Queiroz EF, Marcourt L, Schmiesing A, Hilfiker O, Riezman I, Riezman H, Wolfender JL, Reymond P. (2020). Phosphatidylcholines from Pieris brassicae eggs activate an immune response in Arabidopsis. eLife, 9:e60293. https://doi.org/10.7554/eLife.60293
Stahl E, Hartmann M, Scholten N, Zeier J. (2019). A role for tocopherol biosynthesis in Arabidopsis basal immunity to bacterial infection. Plant Physiology, 181, 1008–1028. https://doi.org/10.1104/pp.19.00618
Full List
Yang Huanjie, Kim Xeniya, Skłenar Jan, Aubourg Sébastien, Sancho-Andrés Gloria, Stahl Elia, Guillou Marie-Charlotte, Gigli-Bisceglia Nora, Tran van Canh Loup, Bender Kyle W, Stintzi Annick, Reymond Philippe, Sánchez-Rodríguez Clara, Testerink Christa, Renou Jean-Pierre, Menke Frank L H, Schaller Andreas, Rhodes Jack, Zipfel Cyril
Stahl Elia, Maier Louis-Philippe, Reymond Philippe
